THE BOVINE BIVARIATE FLOW KARYOTYPE AND PEAK IDENTIFICATION BY CHROMOSOME PAINTING WITH PCR-GENERATED PROBES

A bovine bivariate flow karyotype has been established from a primary fibroblast cell culture carrying a 4;10 Robertsonian translocation. Fr om 27 to 36 populations could be resolved by flow cytometry although t he anticipated number was 31. Separation of chromosomal pairs into two populations explains this hi,oh resolution and confirms the high leve l of heteromorphism previously observed. We used a PARM-PCR (Priming A utorizing Random Mismatches) procedure for the production of paint pro bes from flow-sorted chromosome fractions. These probes were used for chromosome identification by fluorescence in situ hybridization (FISH) on R-banded metaphase spreads. We present the localization of all the bovine chromosome types on the flow karyotype. Twenty-two chromosome types including the translocated chromosome were sorted as pure fracti ons.