CPG ISLAND CLONES FOR CHROMOSOME 11P - A RESOURCE FOR MAPPING AND GENE IDENTIFICATION

A NotI end fragment library has been constructed for human Chromosome (Chr) 11p. Seventy-two clones were mapped to chromosomal subregions by use of somatic cell hybrids. The clones detect 44 different CpG islan ds, and we have isolated cosmid contigs for 36 of them. Extrapolation from the known 11p13 NotI restriction map suggests that every second C pG island from 11p containing a Not site is already represented in the clone collection. By sequence analysis all of the 11p13 clones exhibi t typical features of CpG islands, and cross-species hybridization has been detected with at least one fragment in most cases. The cosmids s erve as valuable linking clones for long-range restriction mapping. Th ey also provide excellent starting material for transcript isolation p rocedures to identify genes on chromosome lip associated with developm ental anomalies and various tumor types. Several transcribed sequences have already been isolated with some of these clones.