ELUCIDATION OF THE FUNCTIONAL-ROLE OF HUMAN 18S RIBOSOMAL-RNA FRAGMENT-980-1061 USING DNA PROBES

Authors
GRAIFER DM ZENKOVA MA MALYGIN AA MATASOVA NB MUNDUS DA KARPOVA GA
Citation
Dm. Graifer et al., ELUCIDATION OF THE FUNCTIONAL-ROLE OF HUMAN 18S RIBOSOMAL-RNA FRAGMENT-980-1061 USING DNA PROBES, Molecular biology, 29(1), 1995, pp. 67-72
Citations number
33
Categorie Soggetti
Biology
Journal title
Molecular biology → ACNP
ISSN journal
00268933
Volume
29
Issue
1
Year of publication
1995
Part
2
Pages
67 - 72
Database
ISI
SICI code
0026-8933(1995)29:1<67:EOTFOH>2.0.ZU;2-1
Abstract
Ten pentadecadeoxynucleotides were used to study several fragments of 18S rRNA. The resulting heteroduplexes were hydrolyzed by RNase H. Onl y four probes bound to fragments 980-994, 987-1001, 1025-1039, and 103 2-1046 of 18S rRNA. None of the probes inhibited tRNA-dependent bindin g of oligo(U) to the 40S subunit. The probes complementary to fragment s 987-1001 and 1025-1039 inhibited translation of poly(U) and formatio n of the pAUG-40S-Met-tRNA(Met)-elF-2-GTP complex, implying that these fragments are involved in ribosome-mRNA interaction.