INTRACELLULAR ACCUMULATION OF OFLOXACIN-LOADED LIPOSOMES IN HUMAN SYNOVIAL FIBROBLASTS

In order to incorporate ofloxacin within liposomes, the reverse-phase evaporation technique was carried out, The liposome lipid matrix consi sted of osphatidylcholine-cholesterol-dihexadecylphosphate (4:3:4 mola r ratio). The liposome formulation presented a mean size of 185 +/- 31 nm and had an encapsulation capacity of 5.3 mu l/mu mol. The liposome formulation was able to deliver ofloxacin into McCoy cells in a great er amount (2.6-fold) than the free drug, improving antibiotic accumula tion.