Jh. Hill et al., RAPID DIFFERENTIATION OF SOYBEAN MOSAIC-VIRUS ISOLATES BY ANTIGENIC SIGNATURE ANALYSIS, Journal of phytopathology, 142(2), 1994, pp. 152-162
Antigenic differences among 14 soybean mosaic virus (SMV) isolates wer
e shown by signature analysis employing a panel of nine well-character
ized monoclonal antibodies. Sets of binding curves were generated by r
eacting aliquots of serial dilutions of leaf extracts from soybean pla
nts infected with each strain of SMV simultaneously with each antibody
in the panel. An iterative alignment procedure allowed comparison of
binding profiles from different assays in which the starting concentra
tions of SMV antigen were unknown. Desiccation of infected soybean lea
f tissue altered antigenic signatures by inducing changes in reactivit
y of epitopes recognized by certain antibodies. The method was useful
for the elucidation of subtle antigenic differences among SMV strains.
The procedure requires a minimum amount of tissue processing, the ana
lysis is rapid and sensitive, and it requires neither purification nor
knowledge of concentration of virus in ground leaf tissue from infect
ed plants.