A HIGHLY STABLE AND SELECTIVE BIOSENSOR USING MODIFIED NICOTINIC ACETYLCHOLINE-RECEPTOR (NACHR)

Authors
ERAY M DOGAN NS REIKEN SR SUTISNA H VANWIE BJ KOCH AR MOFFETT DF SILBER M DAVIS WC
Citation
M. Eray et al., A HIGHLY STABLE AND SELECTIVE BIOSENSOR USING MODIFIED NICOTINIC ACETYLCHOLINE-RECEPTOR (NACHR), Biosystems, 35(2-3), 1995, pp. 183-188
Citations number
12
Categorie Soggetti
Biology
Journal title
Biosystems → ACNP
ISSN journal
03032647
Volume
35
Issue
2-3
Year of publication
1995
Pages
183 - 188
Database
ISI
SICI code
0303-2647(1995)35:2-3<183:AHSASB>2.0.ZU;2-#
Abstract
Methods for developing stable, sensitive and selective bilayer lipid m embrane (BLM)-based biosensors are discussed. Stable BLMs were formed over micromachined polyimide apertures. Selective sensors were made by incorporating nicotinic acetylcholine receptors (nAChRs) modified wit h bispecific antibodies (BsAbs). When two BsAbs, attached to one nAChR , encounter antigen (Ag), channels are blocked. Sensitivity to single Ag molecules would be possible by monitoring closure of individual nAC hRs.