DETECTION OF CIRCULATING TRICHINELLA-SPIRALIS LARVAE BY POLYMERASE CHAIN-REACTION

Citation
P. Uparanukraw et N. Morakote, DETECTION OF CIRCULATING TRICHINELLA-SPIRALIS LARVAE BY POLYMERASE CHAIN-REACTION, Parasitology research, 83(1), 1997, pp. 52-56
Citations number
30
Categorie Soggetti
Parasitiology
Journal title
ISSN journal
09320113
Volume
83
Issue
1
Year of publication
1997
Pages
52 - 56
Database
ISI
SICI code
0932-0113(1997)83:1<52:DOCTLB>2.0.ZU;2-U
Abstract
Several investigators have successfully applied the polymerase chain r eaction to the amplification of DNA from Trichinella spiralis muscle-s tage larvae. We show herein that specific DNA can be amplified from T. spiralis migratory larvae in the blood of experimentally infected mic e. The polymerase chain reaction detected the presence of migratory la rvae in mouse blood from day 5 to day 14 of infection. The technique m ay be applied to human trichinosis, but its diagnostic value will depe nd on the severity and stage of the infection.