Tf. Burke et al., [H-3] MK-801 BINDING TO WELL-WASHED RAT-BRAIN MEMBRANES FOLLOWING CESSATION OF CHRONIC PHENCYCLIDINE TREATMENT, Pharmacology, biochemistry and behavior, 51(2-3), 1995, pp. 435-438
Rats were implanted with osmotic minipumps SC that infused either sali
ne or 10 mg/kg/day phencyclidine (PCP) for 10 days, a regimen that pro
duces dependence to PCP. At the end of this 10-day infusion period, th
e pumps were removed and the rats were sacrificed either immediately o
r at various time points (12 h, 1, 2, and 7 days) after pump removal.
The saturation binding parameters of [H-3]MK-801 were then determined
in well-washed cortical/hippocampal membranes prepared from these rats
. Neither the B-max nor the K-d of [H-3]MK-801 binding in membranes of
PCP-treated rats differed from that determined using membranes from s
aline-treated rats at any time point studied. These results suggest th
at alterations in PCP receptors do not play a major role in the produc
tion of PCP dependence.