N. Wing et al., CULTURAL DEGENERATION IN 2 FUSARIUM SPECIES AND ITS EFFECTS ON TOXIGENICITY AND CULTURAL MORPHOLOGY, Mycological research, 99, 1995, pp. 615-620
Ten isolates each of Fusarium compactum and Fusarium acuminatum subsp.
armeniacum were sub-cultured using single hyphal tips (HT) and single
germinated macroconidia (SM) for ten generations. Cultural degenerati
on to intermediate, mycelial or pionnotal cultures was observed in a l
arge proportion of cultures of both fungi, but pionnotal cultures were
only produced in sub-cultures using SM transfers. Some reversion of i
ntermediate and mycelial cultures to the wild type was observed. Howev
er, pionnotal cultures once formed were stable and did not revert to o
ther cultural types in later generations. The toxicity and trichothece
ne production of degenerate cultures were comparable to those of wild
type cultures. Colony diameters of pionnotal cultures were reduced in
comparison with wild type cultures. The pigmentation of all degenerate
cultures remained consistent with that observed in wild type cultures
. Chlamydospore production was independent of cultural degeneration, a
s all cultural types produced chlamydospores equally well as the paren
tal wild types.