The performance characteristics of a potent new RNase inhibitor have b
een evaluated as have applications for its use in molecular biology PR
IME Inhibitor(TM) is a protein, not related to the commonly available
human placental RNase inhibitors (HPRI). It has a high specific activi
ty enhanced temperature stability, broad reaction pH range and signifi
cantly greater cost-effectiveness than commercial HPRI. PRIME Inhibito
r is suitable for use in vitro transcription, in vitro translation, fi
rst- and second-strand cDNA synthesis, preparation of RNA and mRNA, an
d reverse transcription-polymerase chain reaction.