E-CADHERIN PEPTIDE SEQUENCE RECOGNITION BY ANTI-E-CADHERIN ANTIBODY

Cadherins are calcium dependent glycoproteins involved in hemophilic c ell-cell adhesion. To further elucidate the interaction between cadher ins we have developed an immobilized peptide ELISA to quickly scan the extracellular domains of E-cadherin. Peptides displaying antigenic re activity to anti-E-cadherin antibody are presumably on the surface and thus may be involved in cadherin-cadherin interaction. We have found three peptides from the EC-1 domain which are recognized by the anti-E -cadherin antibody. These peptides are in the two important regions of the EC-1 domain that was deduced hom its secondary structure. (C) 199 5 Academic Press, Inc.