ANTIBODIES TO ICAM-1 PROTECT KIDNEYS IN SEVERE ISCHEMIC REPERFUSION INJURY

ICAM-1 has been implicated in the pathophysiology of ischemic-reperfus ion injury in a number of organs, but its role in mediating severe isc hemic-reperfusion injury in the kidney has not been extensively studie d. Uninephrectomized Sprague Dawley rats were pretreated with either c ontrol monoclonal antibody (mAb) or mAb to ICAM-1 and subjected to 60 min of renal artery occlusion. The serum creatinine, complete blood co unt and kidney histo-pathological damage scores (PDS) (Scale:0-4) were assessed prior to and 24 hours after ischemia. Mean serum creatinine (mg/dl) 24 hours after ischemia was significantly decreased in the ant i-ICAM-1 group (1.38 +/- 0.23, p<0.001) compared to control (2.87 +/- 0.34). PDS was also reduced in anti-ICAM-1 (2.55 +/- 0.20, p<0.05) gro up compared to control (3.35 +/- 0.30). These data demonstrate that bl ocking ICAM-1 significantly mitigates severe ischemic acute renal fail ure, findings which may lead to improved therapy for this condition. ( C) 1995 Academic Press, Inc.