IDENTIFICATION OF MALTING BARLEY VARIETIES BY GENOME ANALYSIS

In this article, we present a useful method to identify malting barley varieties by genome analysis. To identify primer sets that permit the detection of polymorphism, various regions of genes of enzymes or pro teins relevant to brewing were amplified by the polymerase chain react ion (PCR). The PCR-amplified fragments were analyzed by temperature gr adient gel electrophoresis, and then sequenced. We developed the usefu l primer sets from the sequences of alpha-amylase, beta-amylase, beta- glucanase, and B1-hordein. The 10 typical barley varieties tested in t his study were successfully distinguished by the restriction enzyme di gest patterns of products amplified using these primer sets.