PRODUCTION OF RECOMBINANT CYTOCHROME C-551 IN A PSEUDOMONAS-AERUGINOSA MUTANT STRAIN

Authors
ARAI H ZHANG Y SAMBONGI Y IGARASHI Y KODAMA T
Citation
H. Arai et al., PRODUCTION OF RECOMBINANT CYTOCHROME C-551 IN A PSEUDOMONAS-AERUGINOSA MUTANT STRAIN, Journal of fermentation and bioengineering, 79(5), 1995, pp. 489-492
Citations number
19
Categorie Soggetti
Food Science & Tenology","Biothechnology & Applied Migrobiology
Journal title
Journal of fermentation and bioengineering → ACNP
ISSN journal
0922338X
Volume
79
Issue
5
Year of publication
1995
Pages
489 - 492
Database
ISI
SICI code
0922-338X(1995)79:5<489:PORCCI>2.0.ZU;2-I
Abstract
A mutant of Pseudomonas aeruginosa lacking the cytochrome c-551 gene ( nirM) was constructed by marker exchange mutagenesis. When the nirM ge ne was introduced in this strain using a multi-copy expression vector, pHA10, recombinant cytochrome c-551 was produced as a hole-form under aerobic conditions, whereas the expression was not observed when the nirM gene specifically lacking the signal sequence for membrane transl ocation was used.