ISOZYMES FROM CONCHOCELIS OF PORPHYRA SPECIES

The isozyme profiles of Porphyra were examined by means of polyacrylam ide gel electrophoresis to establish a method for distinguishing Porph yra species. The conchocelis of Porphyra used in this experiment inclu ded P. tenera (Minomi), P. tenera (Matsukawa), P.yezoensis f.narawaens is, P.yezoensis (Saga10), P.yezoensis (Green), and P.seriata (Shimabar a). Among the detected enzymes, the electrophoretic profiles of GOT, G DH, PGM, G6PD, ADH, and AK isozymes varied. In particular, the GOT pro file distinguished P. tenera, P.yezoensis, and P.seriata, which was th e wild type, whereas the AK profile differed in all six species. In ad dition, the GDH, PGM and ADH profiles distinguished all six species, w hen combined with those of GOT. In conclusion, the GOT, AK, GDH, PGM a nd ADH profiles obtained by polyacrylamide gel electrophoresis using c onchocelis are thought to be useful for identifying of Porphyra specie s.