RAPID HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF VANCOMYCIN IN HUMAN PLASMA

A rapid simple and robust reversed-phase HPLC method was developed for rapid screening in bioavailability studies or comparative bioequivale nce studies. The method is specific for vancomycin as no interference from acetylsalicylic acid, paracetamol and caffeine was observed. The mean intra-day precision was from 11.7% (low concentration) to 0.3% (h igh concentration) and the within-day precision from 15.0 to 0.3%, det ermined on spiked samples. The accuracy of the method was 106.4-99.8% (intra-day) and 103.5-100.2% (inter-day).