Citation
T. Ito et al., SIMULTANEOUS DETERMINATION OF ASCORBIC-ACID AND DEHYDROASCORBIC ACID IN FISH-TISSUES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography B. Biomedical applications, 667(2), 1995, pp. 355-357
Citations number
5
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography B. Biomedical applications
ISSN journal
15726495
→ ACNP
Volume
667
Issue
2
Year of publication
1995
Pages
355 - 357
Database
ISI
SICI code
Abstract
An high-performance liquid chromatographic method with post-column der
ivatization has been developed for the simultaneous determination of a
scorbic acid (AA) and dehydroascorbic acid (DHAA) in fish tissues. Ext
racted AA and DHAA were separated by a Shim-pack SCR-101H column withi
n 20 min, reacted with sodium hydroxide containing sodium borohydride
and monitored at 300 nm. The detection limits for both AA and DHAA wer
e 0.1 mu g/ml.