ESTROGEN AND PROGESTERONE RECEPTORS IN NORMAL HUMAN ENDOMETRIUM - COMPARISON OF IMMUNOCYTOCHEMICAL ANALYSIS ON FROZEN AND PARAFFIN SECTIONSWITH OR WITHOUT ENZYMATIC PRETREATMENT
Hym. Fung et al., ESTROGEN AND PROGESTERONE RECEPTORS IN NORMAL HUMAN ENDOMETRIUM - COMPARISON OF IMMUNOCYTOCHEMICAL ANALYSIS ON FROZEN AND PARAFFIN SECTIONSWITH OR WITHOUT ENZYMATIC PRETREATMENT, Gynecologic and obstetric investigation, 39(4), 1995, pp. 262-265
Immunocytochemical study in paraffin sections of human endometrium sho
wed that the receptor contents for both oestrogen and progesterone rec
eptors were lower than in the frozen sections although the staining pa
tterns were similar in these two section types. Pretreating the specim
ens with proteolytic enzymes like trypsin, DNase and pronase improved
the oestrogen receptor staining but a better result with progesterone
receptor staining was obtained when no enzymatic pretreatment was appl
ied to the sections.