CRYSTALLIZATION OF A CALCIUM-BINDING EGF-LIKE DOMAIN

Authors
RAO Z HANDFORD PA KNOTT V MAYHEW M BROWNLEE GG STUART D
Citation
Z. Rao et al., CRYSTALLIZATION OF A CALCIUM-BINDING EGF-LIKE DOMAIN, Acta crystallographica. Section D, Biological crystallography, 51, 1995, pp. 402-403
Citations number
12
Categorie Soggetti
Crystallography,"Biochemical Research Methods",Biology
Journal title
Acta crystallographica. Section D, Biological crystallography → ACNP
ISSN journal
09074449
Volume
51
Year of publication
1995
Part
3
Pages
402 - 403
Database
ISI
SICI code
0907-4449(1995)51:<402:COACED>2.0.ZU;2-K
Abstract
Crystals of a calcium-binding epidermal growth factor (EGF)-like domai n of human clotting factor IX suitable for X-ray diffraction analysis have been obtained by vapour diffusion (sitting drop) against 48% PEG 400. The crystals belong to the tetragonal space group P4(3)2(1)2, wit h unit-cell dimensions a = b = 40.3, c = 98.2 Angstrom. The crystals d iffract beyond 1.5 Angstrom resolution and are relatively stable in th e X-ray beam. This is the first reported crystallization of a calcium- binding EGF-like domain.