NONISOTOPIC COMPETITIVE RT-PCR ASSAY TO MEASURE MDR1 GENE-EXPRESSION

We report an original application of competitive reverse transcription -polymerase chain reaction (RT-PCR) for the quantification of MDR1 mRN A in clinical specimens by simultaneous reverse transcription and PCR amplification of cellular RNA with decreasing amounts of an internal s tandard. The competitor RNA shares the same MDR1 primer sequences as t he cellular mRNA, but yields a different-sized PCR product. This allow s resolution of the amplified cDNA fragments after agarose gel electro phoresis and ethidium bromide staining. The concentration of MDR1 mRNA is derived from the ratio between the intensities of the bands corres ponding to the amplified products. We have used this assay to measure MDR1 expression in breast carcinomas and assessed the precision, sensi tivity, and accuracy of the method. Competitive RT-PCR is a simple, hi ghly specific, nonradioactive procedure for the quantification of MDR1 mRNA and is particularly suitable for use in the clinical laboratory.