MULTIENZYME CONTROL SERUM (SERACLEAR-HE) CONTAINING HUMAN ENZYMES FROM ESTABLISHED CELL-LINES AND OTHER SOURCES .2. EVALUATION AS CANDIDATEWORKING ENZYME REFERENCE MATERIAL FOR ALANINE AND ASPARTATE AMINOTRANSFERASES

Seraclear-HE, containing seven enzyme analytes from human sources, was evaluated as an intermethod calibrator for aspartate aminotransferase (AST) and alanine aminotransferase (ALT) to transfer Reference Method values to seven routine methods, including one based on hydrogen pero xide detection for possible unification of values (interlaboratory com parability of data). The commutabilities of AST from erythrocytes and ALT from a hepatoma cell line were studied between the consensus metho ds of Japan Society of Clinical Chemistry (chosen as the Reference Met hods) and each of the automated routine methods at reaction temperatur es of 30 degrees C and 37 degrees C. For AST, calibration of patients' sera with Seraclear-HE decreased average intermethod variation (CV) f rom 12% to 2% for ALT, the decrease was from 20% to 3%. For both enzym es, Seraclear-HE was judged to be commutable between the Reference Met hods and each of the methods investigated. The [imitations for such us e are discussed.