AN IMPROVED METHOD FOR LARGE-SCALE SYNTHESIS OF OLIGONUCLEOTIDES APPLYING THE NPE NPEOC-STRATEGY/

Several oligonucleotides were synthesized in scales up to 60 mu mol in a standard 10 mu mol cartridge on a standard DNA synthesizer. The adv antage of a special phosphoramidite approach using only beta-eliminati ng protecting groups over the commonly practised automated oligonucleo tide synthesis using ammonia-labile blocking groups could be demonstra ted by H-1-NMR-spectroscopy.