I. Toledo et al., REDOX IMBALANCE AT THE START OF EACH MORPHOGENETIC STEP OF NEUROSPORA-CRASSA CONIDIATION, Archives of biochemistry and biophysics, 319(2), 1995, pp. 519-524
The conidiation process of Neurospora crassa is characterized by three
morphogenetic steps: hyphal adhesion, aerial hyphal formation, and pr
oduction of conidia. Total protein oxidation and specific enzyme oxida
tion coincided with an increased oxygen-dependent chemiluminescence an
d indicated the occurrence of a hyperoxidant state at the onset of all
three morphogenetic steps. Oxidation of NAD(P)H and excretion of glut
athione disulfide was detected at the start of hyphae adhesion. Here w
e show that NAD(P)H and glutathione redox imbalance also occurred at t
he beginning of aerial hyphal growth and just before formation of coni
dia in the isolated cell structures. An increased loss and oxidation o
f NAD(P)(H) and glutathione were detected with each morphogenetic tran
sition. These results give further support to our proposal that a hype
roxidant state develops at the start of each of the three morphogeneti
c processes during N. crassa conidiation. (C) 1995 Academic Press, Inc
.