Flavonoid, ascorbic acid and total phenolic content and antioxidant ac
tivity of ''jalapeno'' (Veracruz, Mitla, Tam Mild, Jaloro, Sweet Jalap
eno), ''yellow wax'' [(Hungarian Yellow, Long Hot Yellow, Gold Spike (
hybrid)], ''chile'' (New Mexico-6, Green Chile), ''ancho'' (San Luis A
ncho), and ''serrano'' (Hidalgo) peppers were investigated at green or
yellow stages of maturity. Major pepper flavonoids were quercetin and
luteolin which were present in conjugate forms. Total flavonoid conte
nt varied from none detectable to 800 mg/kg after hydrolysis. ''Chile'
', ''yellow wax'' and ''ancho'' peppers had greater flavonoid and asco
rbic acid contents and antioxidant activities than ''jalapeno'' pepper
s. Sep-Pak C(18)TM bound phenolic compounds, including flavonoids, cor
related well with antioxidant activity (r(2)=0.86). Luteolin had highe
st antioxidant activity followed by capsaicin and quercetin on equimol
ar basis.