MOLECULAR-GENETIC STRAIN IMPROVEMENT FOR THE OVERPRODUCTION OF FUNGALPROTEINS BY FILAMENTOUS FUNGI

During the last 5 years, strain improvement by genetic engineering has been established as a good alternative for traditional methods of str ain improvement such as mutagenesis and genetic recombination. Conside rable success has been achieved in the overproduction of a variety of fungal proteins. However, from the available data a limitation at the level of transcription is evident. A more detailed analysis of this tr anscription limitation was carried out for the overproduction of gluco amylase in A. niger. This analysis revealed that both the site of inte gration of introduced gene copies and the available amount of trans-ac ting regulatory proteins were at the root of the observed limitation. Based on these results, approaches for the construction of a new gener ation of protein-overproducing strains are suggested.