ISOLATION OF SAGI, A NEW HAEIII ISOSCHIZOMER FROM STREPTOCOCCUS-AGALACTIAE

Authors
POCH MT SOMKUTI GA
Citation
Mt. Poch et Ga. Somkuti, ISOLATION OF SAGI, A NEW HAEIII ISOSCHIZOMER FROM STREPTOCOCCUS-AGALACTIAE, Applied microbiology and biotechnology, 43(2), 1995, pp. 282-284
Citations number
13
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Applied microbiology and biotechnology → ACNP
ISSN journal
01757598
Volume
43
Issue
2
Year of publication
1995
Pages
282 - 284
Database
ISI
SICI code
0175-7598(1995)43:2<282:IOSANH>2.0.ZU;2-U
Abstract
A new HaeIII isoschizomer from Streptococcus agalactiae was isolated b y a single-step purification method. The highly active restriction end onuclease, SagI, was free of nonspecific nuclease activity and was sui table for use in molecular biology procedures, The rapid isolation pro cedure may be applicable for the recovery of other restriction endonuc leases from bacteria.