A RAPID BACTOMETER METHOD FOR SCREENING OF BIOCIDES AGAINST SULFATE-REDUCING BACTERIA

A rapid and efficient bactometer method was developed for screening bi ocides against sulfate-reducing bacteria. The method is based on imped ance microbiology principles and uses double-layer API (American Petro leum Institute) agar medium supplemented with 0.1% sodium thioglycolat e as a reducing agent. Compared to the conventional API procedure, whi ch requires 28 days, the present technique takes only 1 day to obtain test results. Excellent linear correlation (r=-0.98) was found between the impedance detection time and log initial cell concentration. The results of the bactometer test were comparable to that of the API bott le test.