ANALYSIS OF RHUBARB BY MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY

A micellar electrokinetic capillary chromatographic method for the sep aration and quantitative determination of aloe-emodin, emodin, and rhe in in rhubarb samples was established. The running electrolyte was a m ixture of 0.025 M 3-(cyclohexylamino)-1-propane sulfonic acid (CAPS) b uffer [containing 0.025 M sodium dodecyl sulfate (SDS)] and acetonicri le (100:10), pH 10.96, with an applied voltage of 12 kV. This method r esolved the three components as well as chrysophanol and physcion from the other constituents present. The technique is simple, rapid, and r eproducible.