PSEUDOTYPE VIRIONS FORMED BETWEEN MOUSE HEPATITIS-VIRUS AND LACTATE DEHYDROGENASE-ELEVATING VIRUS (LDV) MEDIATE LDV REPLICATION IN CELLS RESISTANT TO INFECTION BY LDV VIRIONS
C. Even et Pgw. Plagemann, PSEUDOTYPE VIRIONS FORMED BETWEEN MOUSE HEPATITIS-VIRUS AND LACTATE DEHYDROGENASE-ELEVATING VIRUS (LDV) MEDIATE LDV REPLICATION IN CELLS RESISTANT TO INFECTION BY LDV VIRIONS, Journal of virology, 69(7), 1995, pp. 4237-4244
Infection of cultures of peritoneal macrophages with both lactate dehy
drogenase-elevating virus (LDV) and mouse hepatitis virus (MHV) result
ed in the formation of pseudotype virions containing LDV RNA which pro
ductively infected cells that are resistant to infection by intact LDV
virions but not to infection by MHV. These cells were mouse L-2 and 3
T3-17Cl-1 cells as well as residual peritoneal macrophages from persis
tently LDV-infected mice. Productive LDV infection of these cells via
pseudotype virions was inhibited by antibodies to the MHV spike protei
n or to the MHV receptor, indicating that LDV RNA entered the cells vi
a particles containing the MHV envelope. Simultaneous exposure of L-2
cells to both LDV and MHV resulted in infection by MHV but not by LDV.
The results indicate that an internal block to LDV replication is not
the cause of the LDV nonpermissiveness of many cell types, including
the majority of the macrophages in an adult mouse. Instead, LDV permis
siveness is restricted to a subpopulation of mouse macrophages because
only these cells possess a surface component that acts as an LDV rece
ptor.