Production of proteolytic enzyme in a bubble column bioreactor was car
ried out using the microorganism Rhizopus oryzae. Optimization of the
production conditions were done adopting the EVOP (evolutionary operat
ion technique). Studies on two variable and three variable parameters
were carried out. The variable parameters were air flow rate, aeration
time and incubation period. 8 hours aeration time per day, 1.66 vvm a
ir flow rate and 2 days incubation was found to be optimum.