P. Kintz et P. Mangin, SIMULTANEOUS DETERMINATION OF OPIATES, COCAINE AND MAJOR METABOLITES OF COCAINE IN HUMAN HAIR BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY (GC MS)/, Forensic science international, 73(2), 1995, pp. 93-100
A procedure is presented for the simultaneous identification and quant
ification of morphine (MOR), codeine (GOD), ethylmorphine (EM), 6-mono
acetylmorphine (6-MAM), cocaine (COG), benzoylecgonine (BZE), ecgonine
methylester (EME) and cocaethylene (CE), contained in the hair of opi
ates and cocaine addicts. The method involves decontamination in dichl
oromethane, pulverization in a hall mill, heat-acid hydrolysis, additi
on of deuterated internal standards, liquid-liquid extraction and gas
chromatography/mass spectrometry (GC/MS) after silylation. The limit o
f detection (LOD) was similar to 0.1-0.8 ng/mg for each drug, using a
30-mg hair sample. The method is reproductible, with a coefficient of
variation (CV) of similar to 8-17%. Cocaine and 6-monoacetylmorphine w
ere the major compounds detected in cases of cocaine (14 cases) and he
roin (68 cases) intake. Concentrations were in the range 0.4-78.4 ng/m
g (COG), 0.0-36.3 ng/mg (BZE), 0.0-1.6 ng/mg (EME), 0.0-2.1 ng/mg (CE)
, 0.0-84.3 ng/mg (6-MAM), 0.2-27.1 ng/mg (MOR) and 0.1-19.6 ng/mg (GOD
). An application in forensic sciences, involving multi-sectional anal
ysis, is given.