The koji ratio (rice for koji/rice for kake) had a significant influen
ce on the formation of higher alcohols and aromatic esters by yeast (S
accharomyces cerevisiae) during rice shochu fermentation. The concentr
ations of acetaldehyde, isobutyl alcohol, isoamyl alcohol and beta-phe
nylethyl alcohol in shochu increased with decreasing koji ratio. There
was an optimum koji ratio for the formation of isoamyl acetate and be
ta-phenylethyl acetate. The formol titration value in matured mash was
about 3 ml at the optimum koji ratio.