AN ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY STUDY OF COPPER ADDUCTS OF PROTONATED UBIQUITIN

Authors
JIAO CQ FREISER BS CARR SR CASSADY CJ
Citation
Cq. Jiao et al., AN ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY STUDY OF COPPER ADDUCTS OF PROTONATED UBIQUITIN, Journal of the American Society for Mass Spectrometry, 6(6), 1995, pp. 521-524
Citations number
19
Categorie Soggetti
Chemistry Physical","Chemistry Analytical",Spectroscopy
Journal title
Journal of the American Society for Mass Spectrometry → ACNP
ISSN journal
10440305
Volume
6
Issue
6
Year of publication
1995
Pages
521 - 524
Database
ISI
SICI code
1044-0305(1995)6:6<521:AEMSOC>2.0.ZU;2-Y
Abstract
A study of the addition of Cu(II) to a ubiquitin electrospray solution shows that the copper ion in the ubiquitin remains doubly charged and displaces two protons on the protonated protein molecule. This observ ation indicates a chelating bond between the protein and the Cu(II) sp ecies. The addition of Cu(I) also was studied and significant intensit y was observed for adducts with up to four Cu(I) species attached, wit h each Cu(I) bonded to one basic site on the protein.