Two strains of halophilic archaebacteria, growing in a medium containi
ng from 10 to 25% NaCl, were isolated from the brines of the Kalamkass
(Mangyshlak) oil field. Both strains are extremely halophilic archaeb
acteria according to the complex of their phenotypic properties. Strai
n M-11 was identified as Haloferax mediterranei on the basis of the co
mposition of polar lipids and DNA-DNA homology. The composition of pol
ar lipids and 16S rRNA sequence of strain M-ls allowed us to assign it
to the genus Haloferax. This strain differs from the approved species
of the genus Haloferax, H.volcanii, and H.mediterranei. However, to d
escribe it as a new species, additional investigations are necessary.