P. Samadder et al., 1-O-OCTADECYL-2-O-METHYL-GLYCEROPHOSPHOSCHOLINE INHIBITS THE ADHESIONOF RAS-TRANSFORMED FIBROBLASTS TO HUMAN ENDOTHELIAL-CELLS, Oncology Reports, 2(4), 1995, pp. 647-650
The adhesion of tumor cells to the endothelium is a key step in haemat
ogenic metastasis. Because alkyllysophospholipids decrease tumor metas
tasis, we investigated the effect of 1-O-octadecyl-2-O-methyl-glycerop
hosphocholine (ET18-OCH3) on the adhesion of two highly metastatic ras
-transformed 10T1/2 cell lines, CIRAS-2 and CIRAS-3, to confluent endo
thelial cells. The order of adhesion to the endothelial cells was CIRA
S-2 > 10T1/2 > CIRAS-3. RGD peptides were unable to inhibit the bindin
g of the cells suggesting that adhesion was not mediated by fibronecti
n or vitronectin. Treatment of the cell lines with 20 mu M ET18-OCH3 f
or 24 h decreased the adhesion of CIRAS-2, CIRAS-3 and 10T1/2 to the e
ndothelial cells by 50, 43 and 36% respectively. Thus interference in
the adhesion of tumor cells to the endothelium could contribute to dec
reased metastasis.