THE BLOCKAGE OF THE HUMAN TRANSFERRIN RECEPTOR BY A MONOCLONAL-ANTIBODY, EA.3, INDUCES GROWTH-INHIBITION IN LEUKEMIA-CELL LINES

Authors
VALENTINI M GREGORINI A BARTOLUCCI M PORCELLINI A PAPA S
Citation
M. Valentini et al., THE BLOCKAGE OF THE HUMAN TRANSFERRIN RECEPTOR BY A MONOCLONAL-ANTIBODY, EA.3, INDUCES GROWTH-INHIBITION IN LEUKEMIA-CELL LINES, European journal of histochemistry, 38, 1994, pp. 61-67
Citations number
12
Categorie Soggetti
Cell Biology
Journal title
European journal of histochemistry → ACNP
ISSN journal
1121760X
Volume
38
Year of publication
1994
Supplement
1
Pages
61 - 67
Database
ISI
SICI code
1121-760X(1994)38:<61:TBOTHT>2.0.ZU;2-2
Abstract
In our program to produce monoclonal antibodies against hemopoietic fu nctional antigens we have obtained an hybridoma cell line EA.3 reactin g with human transferrin receptor. This monoclonal antibody is an IgM that displays ''in vitro'' antiproliferative activity against leukemic cells due to its competition with transferrin on the receptor. Cell c ytotoxicity was demonstrated either with the blockage of BrdU incorpor ation in HL-60 cells and with leukemic CFU inhibition. In contrast, K5 62 cells were insensitive to the blockage of the transferrin receptor induced by this MAb.