INSULIN-MEDIATED INCREASE IN BLOOD-FLOW IS IMPAIRED IN THE ELDERLY

It has recently been recognized that the ability of insulin to augment blood flow is reduced in insulin-resistant conditions such as obesity and noninsulin-dependent diabetes mellitus. Normal aging is character ized by resistance to insulin-mediated glucose uptake. We undertook th e following studies with the hypothesis that the resistance to insulin -mediated glucose uptake that occurs with aging is caused in part by a reduction in insulin-mediated blood flow. These experiments were cond ucted on healthy young (n = 13; age, 24 +/- 1 yr; body mass index: 22. 2 +/- 0.6 kg/m(2)) and old (n = 13; age, 77 +/- 1 yr; body mass index: 24.2 +/- 0.5 kg/m(2)) subjects. Each subject underwent two studies. I n the control study, saline was infused for 4 h. In the euglycemic cla mp study, insulin was infused for 4 h at a rate of 40 mu/m(2) . min in the young subjects and 34 mU/m(2) . min in the old subjects. Blood sa mples were taken, and calf blood flow was measured using venous occlus ion plethysmography at regular intervals in each study. Basal calf blo od now was lower in the elderly (young subjects: 1.51 +/- .08 mL/100 m L tissue per min; old subjects: 1.15 +/- 0.07 mL/100 mt tissue per min , P < 0.002). During the euglycemic clamp studies, steady-state insuli n and glucose values were similar in the two age groups. Glucose dispo sal rates were significantly higher in the young subjects (P = 0.01 by analysis of variance). Mean arterial pressure values were significant ly higher in the elderly (P < 0.001 by analysis of variance) throughou t the clamp, but there was no significant change over time in either a ge group. The mean incremental blood flow rate at steady-state (180-24 0 min) was significantly higher in the young subjects (0.76 +/- 0.23 m L/100 mt tissue per min) than in the old subjects (0.05 +/- 0.09 mL/10 0 mt tissue per min, P < 0.01). There was a significant correlation be tween steady-state glucose disposal rate values and incremental blood flow rates in the young subjects (r = 0.59, P < 0.05) but not in the o ld subjects (r = 0.21, P = NS). We conclude that normal aging is chara cterized by an impairment in the ability of insulin to modulate blood flow, which may contribute in part to the insulin resistance of aging.