Based on the spontaneous migration of radioactive tracer from the post
erior vaginal fornix to the ovaries and peritoneal cavity, several att
empts were made to assess hystero-salpingo scintigraphy (HSS). The low
acceptance rate by sterile women of routine investigation of tubal fu
nction may be due to a fear of radiation exposure and unpleasant exami
nation procedures. Our protocol for HSS adopts a low dose of radioacti
ve tracer (0.2-0.3 mCi), a defined mode of application (between the ex
ternal and internal os of the cervical canal) and a short imaging time
(60 min). From 1990 to 1992, we investigated 60 fallopian tubes in 32
sterile women by HSS, hystero-salpingography (HSG) and/or chromopertu
bation during laparoscopy (LPSC). The results of HSG and HSS correspon
ded in 15 of 49 fallopian tubes, LPSC and HSS in 9 of 24. None of the
32 patients had become pregnant naturally during the average observati
on period of 17 months. Two patients became pregnant after in-vitro fe
rtilization. HSS, performed according to our protocol, causes less pai
n and results in a lower dose of radiation than HSG (about 50%). It is
well accepted by patients and is easy to perform. As an investigation
of tubal function, HSS may serve as an additional examination techniq
ue in cases of presumed tubal sterility.