Artemisia annua L. seeds from North Viet Nam were germinated and conti
nuously propagated by tissue culture technique. Three-week-old plantle
ts were transplanted from the laboratory into the field under tropical
climatic conditions and assayed for artemisinin at different stages o
f growth. The plants had short stature (100 cm), early flowering (14 w
eeks), short growth span (18-20 weeks), variable artemisinin content a
nd produced no seeds. The highest artemisinin yield was found in the l
eaves of twelve (0.13 - 0.31%) to thirteen (0.12-0.39%) weeks old plan
ts during pre-flowering periods. Plantlets in the test tubes were rand
omly selected and cloned. Statistical comparison of the artemisinin co
ntent from the various clones indicated that they may be grouped as hi
gh (0.41 - 0.42%), moderate (0.25 - 0.26%), and low (0.13%) artemisini
n yielders.