PERMEABILIZATION OF DATURA-INNOXIA HAIRY ROOTS FOR RELEASE OF STORED TROPANE ALKALOIDS

Authors
BOITELCONTI M GONTIER E LABERCHE JC DUCROCQ C SANGWANNORREEL BS
Citation
M. Boitelconti et al., PERMEABILIZATION OF DATURA-INNOXIA HAIRY ROOTS FOR RELEASE OF STORED TROPANE ALKALOIDS, Planta medica, 61(3), 1995, pp. 287-290
Citations number
20
Categorie Soggetti
Pharmacology & Pharmacy","Plant Sciences
Journal title
Planta medica → ACNP
ISSN journal
00320943
Volume
61
Issue
3
Year of publication
1995
Pages
287 - 290
Database
ISI
SICI code
0032-0943(1995)61:3<287:PODHRF>2.0.ZU;2-P
Abstract
The effects of tween 20 as permeabilizing agent on tropane alkaloids f rom Datura innoxia Mill. hairy roots have been studied. For various tw een 20 concentrations both hyoscyamine and scopolamine accumulated in the culture medium. Plant material viability could be preserved after a 24 hours-2% tween 20 concentration treatment. The time-course study of alkaloid release showed that the maximum of excretion occurred afte r a 20 hour contact with tween 20. At that time, a concentration of hy oscyamine superior to 25 mg/l was detected in the medium.