We conducted a set of in situ incubations to evaluate patterns of N av
ailability among dominant land uses in the shortgrass steppe region of
Colorado, USA, and to assess recovery of soil fertility in abandoned
fields. Replicated 30 d incubations were performed in 3 sets of native
(never cultivated), abandoned (cultivated until 1937), and currently
cultivated, fallow fields. Net N mineralization and the percentage of
total N that was mineralized increased in the order: native, abandoned
, cultivated Higher soil water content in fallow fields is the most li
kely reason for greater mineralization in cultivated fields, while hig
her total organic C and C/N ratios in native and abandoned fields may
explain differences in mineralization between these land uses. Recover
y of soil organic matter in abandoned fields appears to involve accumu
lation of soil C and N under perennial plants, but probable methodolog
ical artifacts complicate evaluation of the role of individual plants
in recovery of N availability. Higher N mineralization and turnover in
cultivated fields may make them more susceptible to N losses; recover
y of N cycling in abandoned fields appears to involve a return to slow
er N turnover and tighter N cycling similar to native shortgrass stepp
e.