INHIBITION OF GLYCOGEN-SYNTHASE KINASE-3 BY INSULIN IN CULTURED HUMANSKELETAL-MUSCLE MYOBLASTS

Authors
BORTHWICK AC WELLS AM ROCHFORD JJ HUREL SJ TURNBULL DM YEAMAN SJ
Citation
Ac. Borthwick et al., INHIBITION OF GLYCOGEN-SYNTHASE KINASE-3 BY INSULIN IN CULTURED HUMANSKELETAL-MUSCLE MYOBLASTS, Biochemical and biophysical research communications, 210(3), 1995, pp. 738-745
Citations number
24
Categorie Soggetti
Biology,Biophysics
Journal title
Biochemical and biophysical research communications → ACNP
ISSN journal
0006291X
Volume
210
Issue
3
Year of publication
1995
Pages
738 - 745
Database
ISI
SICI code
0006-291X(1995)210:3<738:IOGKBI>2.0.ZU;2-W
Abstract
The acute effects of insulin on the activity of glycogen synthase kina se 3 (GSK-3) have been investigated both in the rat L6 muscle cell lin e and in cultured human myoblasts, The alpha and beta isoforms of GSK- 3 are present in both cell types,with the beta isoform being predomina nt in the human cells, insulin causes a rapid inactivation of both iso forms in both cell lines, with 50% inactivation being observed in the human myoblasts. (C) 1995 Academic Press, Inc.