PEPTIDYL DIPEPTIDASE A-CATALYZED METABOLISM OF DELTA-SLEEP-INDUCING PEPTIDE IN BOVINE BRAIN MICROVESSEL ENDOTHELIAL-CELLS - A CELL-CULTURE MODEL OF THE BLOOD-BRAIN-BARRIER
Pf. Augustijns et al., PEPTIDYL DIPEPTIDASE A-CATALYZED METABOLISM OF DELTA-SLEEP-INDUCING PEPTIDE IN BOVINE BRAIN MICROVESSEL ENDOTHELIAL-CELLS - A CELL-CULTURE MODEL OF THE BLOOD-BRAIN-BARRIER, Biochemical and biophysical research communications, 210(3), 1995, pp. 987-994
Previous studies have shown that the metabolism of delta sleep-inducin
g peptide (DSIP) in the blood-brain barrier (BBB) is catalyzed by amin
opeptidases. In this study, we have shown that peptidyl dipeptidase A
in cultured bovine brain microvessel endothelial cells (BBMEC), a mode
l of the BBB, and a purified form of this enzyme can also metabolize D
SIP by sequential hydrolyses of dipeptides or tripeptides from the car
boxyl terminus of this nonapeptide. Both the dipeptidase and tripeptid
ase activity associated with peptidyl dipeptidase A can be inhibited b
y captopril. Total stabilization of DSIP to metabolism in BBMEC could
be achieved by inclusion of an inhibitor of peptidyl dipeptidase A (e.
g., captopril) and an inhibitor of aminopeptidases (e.g., bestatin). (
C) 1995 Academic Press, Inc.