DNA SEPARATIONS BY SLAB GEL AND CAPILLARY ELECTROPHORESIS - THEORY AND PRACTICE

In this review we evaluate existing experimental methods for the elect rophoretic size separation of nucleic acids, including both slab gel e lectrophoresis and capillary electrophoresis (CE). First, we briefly d iscuss some important applications of DNA electrophoresis in molecular biology. We then focus on the theory and the practice of DNA separati ons by slab gel electrophoresis and CE, emphasizing the different prac tical limitations of the two techniques. In particular, we review new developments in CE, a technique which is evolving rapidly.