K. Sathasivam et al., SEX DETERMINATION IN THE DOMESTIC PIG BY DNA AMPLIFICATION USING THE HMG-BOX SEQUENCE, Animal reproduction science, 38(4), 1995, pp. 321-326
We describe a rapid, reliable method for the sexing of domestic pigs (
Sus scrofa domestica) by amplification of Y-chromosome specific sequen
ces in male genomic DNA using the polymerase chain reaction (PCR). Oli
gonucleotide primers were selected from a conserved sequence motif, th
e HMG box, in the sequence of porcine Sry. These primers permit amplif
ication of a defined 146 bp fragment only from male-specific genomic D
NA. As a control, autosomal-specific primers were also used in the PCR
reactions, recognising a sequence from a porcine homeobox gene that i
s amplifiable in genomic DNA from both males and females. This sensiti
ve method will find application in the sexing of biopsied, pre-implant
ation embryos and foetuses used for the derivation of embryonic cell l
ines and germ-cell derivatives, for use in the new biotechnologies; an
d in the analysis of male-specific sequences in intersex pigs.