UNDEREVALUATION OF COMPLEX-I ACTIVITY BY THE DIRECT ASSAY OF NADH COENZYME-Q REDUCTASE IN RAT-LIVER MITOCHONDRIA

Authors
LENAZ G FATO R GENOVA ML FORMIGGINI G CASTELLI GP BOVINA C
Citation
G. Lenaz et al., UNDEREVALUATION OF COMPLEX-I ACTIVITY BY THE DIRECT ASSAY OF NADH COENZYME-Q REDUCTASE IN RAT-LIVER MITOCHONDRIA, FEBS letters, 366(2-3), 1995, pp. 119-121
Citations number
15
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
366
Issue
2-3
Year of publication
1995
Pages
119 - 121
Database
ISI
SICI code
0014-5793(1995)366:2-3<119:UOCABT>2.0.ZU;2-L
Abstract
We have shown that the rate of NADH-coenzyme Q reductase in rat liver mitochondria, assayed using the decyl-ubiquinone analog DB, is underev aluated, probably as a result of its low water solubility. In view of drawbacks encountered using other more soluble accepters in this syste m, we demonstrate that the most reliable assay of the physiological ra te of CoQ reduction by Complex I is the indirect calculation from the total rate of NADH oxidation and the rate of ubiquinol oxidation, usin g the pool equation of Kroger and Klingenberg [(1973) Eur. J. Biochem. 34, 358-368].