HPLC SEPARATION OF 2 NOVEL DIKETOPIPERAZINE ISOMERS ON POLYVINYL-ALCOHOL FUNCTIONALIZED SILICA-GEL

Authors
MIERZWA R PATEL M CHU M
Citation
R. Mierzwa et al., HPLC SEPARATION OF 2 NOVEL DIKETOPIPERAZINE ISOMERS ON POLYVINYL-ALCOHOL FUNCTIONALIZED SILICA-GEL, Journal of liquid chromatography, 18(10), 1995, pp. 2019-2027
Citations number
9
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of liquid chromatography → ACNP
ISSN journal
01483919
Volume
18
Issue
10
Year of publication
1995
Pages
2019 - 2027
Database
ISI
SICI code
0148-3919(1995)18:10<2019:HSO2ND>2.0.ZU;2-N
Abstract
An HPLC method is described for the analytical separation of two novel diketopiperazine isomers isolated from a fungal culture, Tolypocladiu m sp. (SCF-0729). Separation was achieved on a highperformance polyvin yl alcohol silica gel column with a 1-chlorobutane: methanol gradient system. Selectivity, retention and resolution obtained with this suppo rt were all highly reproducible. Direct scale up of analytical methodo logy allowed for the preparative separation of this isomeric pair.