Pr. Shepherd et al., INHIBITORS OF PHOSPHOINOSITIDE 3-KINASE BLOCK EXOCYTOSIS BUT NOT ENDOCYTOSIS OF TRANSFERRIN RECEPTORS IN 3T3-L1 ADIPOCYTES, Biochemical and biophysical research communications, 211(2), 1995, pp. 535-539
The role of PI 3-kinase in transferrin receptor recycling was investig
ated in 3T3-L1 adipocytes. Wortmannin, a specific PI 3-kinase inhibito
r, blocked insulin stimulated recruitment of transferrin receptors to
the plasma membrane (IC50 approximate to 5-15 nM) with a similar poten
cy to its inhibitory effects on PI 3-kinase and glucose transport. Wor
tmannin also blocked insulin stimulated recruitment of IGF-2 receptors
to the cell surface. However, wortmannin (up to 200 nM) and another P
I 3-kinase inhibitor, LY294002 (3 mu g/ml), did not block transferrin
receptor endocytosis. This demonstrates that while PI 3-kinase may be
necessary for insulin stimulated exocytosis, it is not necessary for e
ndocytosis of transferrin receptors. (C) 1995 Academic Press, Inc.