CALCULATED EFFECTS OF THE CHEMICAL ENVIRONMENT OF 2-PHOSPHO-D-GLYCERATE ON THE PK(A) OF ITS CARBON-2 AND CORRELATIONS WITH THE PROPOSED MECHANISM OF ACTION OF ENOLASE

Authors
HILAL SH BREWER JM LEBIODA L CARREIRA LA
Citation
Sh. Hilal et al., CALCULATED EFFECTS OF THE CHEMICAL ENVIRONMENT OF 2-PHOSPHO-D-GLYCERATE ON THE PK(A) OF ITS CARBON-2 AND CORRELATIONS WITH THE PROPOSED MECHANISM OF ACTION OF ENOLASE, Biochemical and biophysical research communications, 211(2), 1995, pp. 607-613
Citations number
25
Categorie Soggetti
Biology,Biophysics
Journal title
Biochemical and biophysical research communications → ACNP
ISSN journal
0006291X
Volume
211
Issue
2
Year of publication
1995
Pages
607 - 613
Database
ISI
SICI code
0006-291X(1995)211:2<607:CEOTCE>2.0.ZU;2-I
Abstract
The mechanism of action of enolase requires drastic lowering of the pK of the a carbon of the substrate to enable removal of the alpha hydro gen. Calculations suggest this is done primarily by rotation of the ca rboxyl (''carbon-1'') of the substrate around the carbon-1 carbon-2 bo nd by 90 degrees so that its oxygen atoms lie in the plane formed by t he three carbon atoms and secondarily by effectively neutralizing the negative charges on the carboxylate and phosphate. These predictions a re in very good agreement with the proposed mechanism of action of eno lase which is based on X-ray crystallographic data. (C) 1995 Academic Press, Inc.