THE EFFECT OF SOLID MEDIUM COMPOSITION ON GROWTH AND SPORULATION OF STREPTOMYCES-CLAVULIGERUS - SPORE VIABILITY DURING STORAGE AT -DEGREES-C(4)

The effect of solid medium composition and pH on growth and sporulatio n of Streptomyces clavuligerus was studied in Petri dish and slant cul tures at 30 degrees C. The extent of sporulation was observed under th e microscope and the number of viable spores per slant was counted by serial dilution. Abundant aerial mycelium and sporulation were achieve d with some of the media tested in this work. In Tris/HCl buffer (0.05 M, pH 7.2), containing 0.1% Tween 80, spores retained 28%, 17% and 2% viability at +4 degrees C after 1,9 and 17.5 weeks, respectively.